SRA

Through rational mutagenesis of the RAG1 and RAG2 proteins, we have generated RAG complex that is highly active for transposition in cells. The mutant RAG complex can transpose DNA fragments flanked by 12/23RSSs into the genome. We used a high throughput sequencing method to measure and map the transposition events mediated by this mutant RAG complex into the genome of human HEK293T cells. This study provides insight into the evolutionary events that allowed the RAG transposase to evolve into the RAG recombinase now found in jawed vertebrates and also reveals target preferences of RAG-mediated transposition.