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SRX4704171: X54 Control
1 ILLUMINA (Illumina HiSeq 2500) run: 32.7M spots, 6.5G bases, 2.5Gb downloads

Design: After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. After agarose gel electrophoresis, the suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqTM 2500 or other sequencer when necessary.
Submitted by: Zhejiang University
Study: Transcriptome analysis of barley epidermal cells under drought stresses
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Comparative transcriptomic study with two wild barley genotypes X5 & X54
Sample: XZ54_C
SAMN10077048 • SRS3790141 • All experiments • All runs
Name: 3
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 32.7M spots, 6.5G bases, 2.5Gb
Run# of Spots# of BasesSizePublished


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