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SRX220564: Puccinia striiformis f.sp. tritici race PST-43
1 ILLUMINA (Illumina HiSeq 2000) run: 9.5M spots, 1.9G bases, 1.3Gb downloads

Design: Genomic DNA was extracted for each race from dried urediniospores using the CTAB method. A single DNA library was prepared from 5 *g of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Primers with 6 nt long barcodes were used. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).
Submitted by: UC DAVIS
Study: Puccinia striiformis f. sp. tritici race PST-43 shotgun sequencing
show Abstracthide Abstract
The fungus Puccinia striiformis is the causal pathogen of stripe rust in wheat. New highly virulent Pst races appeared at the beginning of this century and spread rapidly causing significant yield losses in wheat production worldwide. Race PST-43 was isolated in the US in 1990 and is virulent on wheat varieties carrying the resistance genes Yr2, Yr6, Yr20, and Yr21.
Sample: Puccinia striiformis f. sp. tritici race PST-43
SAMN01818050 • SRS376448 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: WGS
Selection: unspecified
Layout: PAIRED
Spot descriptor:
forward101  reverse

Runs: 1 run, 9.5M spots, 1.9G bases, 1.3Gb
Run# of Spots# of BasesSizePublished


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