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SRX220056: Transcriptome Sequencing of cold-stress Jatropha curcas L.
1 ILLUMINA (Illumina HiSeq 2000) run: 27.6M spots, 5G bases, 3.1Gb downloads

Design: Tissue samples from three cold treatments and the control seedlings of Jatropha curcas L., were equally mixed for RNA preparation. Total RNA was extracted using a TRIzol reagent (Invitrogen) following the manufacture’s protocol. The concentration of the RNA was determined with Qubit Fluorometer, and the quality was checked by Agilent 2100 Bioanalyzer and 1% (w/v) agarose gel electrophoresis. By using oligo(dT)-attached magnetic beads, poly(A)-containing mRNAs were enriched from the total RNA extracts. Fragmentation buffer was added for interrupting mRNA to short segments, which were taken as the templates to synthesize cDNAs by random hexamer-primers. Then, the short DNA products were purified with QiaQuick PCR extraction kit and ligated with sequencing adapters. By agarose gel electrophoresis, suitable fragments with 200-700bp were selected for PCR amplification and sequencing templates by the Illumina Hiseq™ 2000 RNA-seq system.
Submitted by: YUNNAN NORMAL UNIVERSITY
Study: Jatropha curcas L. gene expression under cold stress
show Abstracthide Abstract
Jatropha curcas L., as an important renewable bioenergy plant, has gradually attracted increased interest for biodiesel, due to its high oil content in seed and grown on non-agricultural lands. Cold stress, main limiting factor, impairs the cultivation and distribution of J.curcas. In this study, a powerful deep high-throughput sequencing approach named Illumina Hiseq™ 2000 RNA-seq was exploited for transcriptome sequencing analysis of J. curcas from a mixed sampling of the untreated and cold-treated seedlings. The results allowed us to decipher the key genes related to the biosynthesis of oils and secondary metabolites especially of the diterpenes such as phorbol esters, as well as those coding for cold tolerance in this sequence library.
Sample: General Sample from untreated and cold-treated seedlings of Jatropha curcas L.
SAMN01894327 • SRS387632 • All experiments • All runs
Organism: Jatropha curcas
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PCR
Layout: PAIRED
Spot descriptor:
forward91  reverse

Runs: 1 run, 27.6M spots, 5G bases, 3.1Gb
Run# of Spots# of BasesSizePublished
SRR65319827,556,0715G3.1Gb2015-07-22

ID:
307193

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