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SRX3524647: Day 0 biological replicate 1
1 ILLUMINA (Illumina HiSeq 2000) run: 20.1M spots, 2G bases, 912.6Mb downloads

Design: Large molecular weight RNAs were differentially isolated from the small molecular weight RNA fraction using the SPLIT RNA extraction kit (Lexogen, Vienna, Austria) as per the manufacturer s instructions. RNA quality was evaluated using the Agilent RNA 6000 Nano Kit on a Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA, USA). Only RNA samples with an integrity number > 7.0 were kept for RNA-seq library construction. Each sample was quantified using a Nanodrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and, prior to constructing RNA-seq libraries, 1.5 g of RNA was enriched in poly-A RNA using magnetic beads with poly-T oligonucleotides. Enriched poly-A RNA was used to construct libraries using the Illumina TrueSeq RNA sample prep kit v2 (llumina, San Diego, Ca, USA) as per the manufacturer s instructions except that the RNA fragmentation step was performed during six minutes.
Submitted by: Laval university
Study: Differential expression profiling of microspores during the early stages of isolated microspore culture in barley using the embryogenic-responsive cultivar Gobernadora
show Abstracthide Abstract
mRNA library from Illumina TrueSeq; Barley (Hordeum vulgare ssp. vulgare; cv. Gobernadora) starting material in barley androgenesis: uninucleate and haploid microspores (Day 0), microspore after stress pre-treatment (Day 2) and microspore after three days in embryogenesis culture (Day 5).
Sample: Day 0 biological replicate 1
SAMN08280990 • SRS2800831 • All experiments • All runs
Organism: Hordeum vulgare
Name: D0_rep.1_raw_data
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 20.1M spots, 2G bases, 912.6Mb
Run# of Spots# of BasesSizePublished


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