Day 0 biological replicate 4 - SRA

SRX3524650: Day 0 biological replicate 4
1 ILLUMINA (Illumina HiSeq 2000) run: 24.5M spots, 2.4G bases, 1.1Gb downloads

Design: Large molecular weight RNAs were differentially isolated from the small molecular weight RNA fraction using the SPLIT RNA extraction kit (Lexogen, Vienna, Austria) as per the manufacturer s instructions. RNA quality was evaluated using the Agilent RNA 6000 Nano Kit on a Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA, USA). Only RNA samples with an integrity number > 7.0 were kept for RNA-seq library construction. Each sample was quantified using a Nanodrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and, prior to constructing RNA-seq libraries, 1.5 g of RNA was enriched in poly-A RNA using magnetic beads with poly-T oligonucleotides. Enriched poly-A RNA was used to construct libraries using the Illumina TrueSeq RNA sample prep kit v2 (llumina, San Diego, Ca, USA) as per the manufacturer s instructions except that the RNA fragmentation step was performed during six minutes.

Submitted by: Laval university

Study: Differential expression profiling of microspores during the early stages of isolated microspore culture in barley using the embryogenic-responsive cultivar Gobernadora

PRJNA428086 • SRP127768 • All experiments • All runs

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mRNA library from Illumina TrueSeq; Barley (Hordeum vulgare ssp. vulgare; cv. Gobernadora) starting material in barley androgenesis: uninucleate and haploid microspores (Day 0), microspore after stress pre-treatment (Day 2) and microspore after three days in embryogenesis culture (Day 5).

Sample: Day 0 biological replicate 4

SAMN08280993 • SRS2800834 • All experiments • All runs

Organism: Hordeum vulgare

Library:

Name: D0_rep.4_raw_data

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 24.5M spots, 2.4G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR6433025	24,454,912	2.4G	1.1Gb	2018-02-28

ID:: 4897426

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