U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX3524660: Day 5 biological replicate 4
1 ILLUMINA (Illumina HiSeq 2000) run: 24.6M spots, 2.5G bases, 1.1Gb downloads

Design: Large molecular weight RNAs were differentially isolated from the small molecular weight RNA fraction using the SPLIT RNA extraction kit (Lexogen, Vienna, Austria) as per the manufacturer s instructions. RNA quality was evaluated using the Agilent RNA 6000 Nano Kit on a Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA, USA). Only RNA samples with an integrity number > 7.0 were kept for RNA-seq library construction. Each sample was quantified using a Nanodrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and, prior to constructing RNA-seq libraries, 1.5 g of RNA was enriched in poly-A RNA using magnetic beads with poly-T oligonucleotides. Enriched poly-A RNA was used to construct libraries using the Illumina TrueSeq RNA sample prep kit v2 (llumina, San Diego, Ca, USA) as per the manufacturer s instructions except that the RNA fragmentation step was performed during six minutes.
Submitted by: Laval university
Study: Differential expression profiling of microspores during the early stages of isolated microspore culture in barley using the embryogenic-responsive cultivar Gobernadora
show Abstracthide Abstract
mRNA library from Illumina TrueSeq; Barley (Hordeum vulgare ssp. vulgare; cv. Gobernadora) starting material in barley androgenesis: uninucleate and haploid microspores (Day 0), microspore after stress pre-treatment (Day 2) and microspore after three days in embryogenesis culture (Day 5).
Sample: Day 5 biological replicate 4
SAMN08281001 • SRS2800838 • All experiments • All runs
Organism: Hordeum vulgare
Library:
Name: D5_rep.4_raw_data
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 24.6M spots, 2.5G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR643301524,578,4042.5G1.1Gb2018-02-28

ID:
4897436

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...