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SRX3109094: HSMA33MX stool WGS
1 ILLUMINA (Illumina HiSeq 2000) run: 213,433 spots, 39.2M bases, 14.5Mb downloads

Design: Metagenomic DNA samples were quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50 pg/microliter. Illumina sequencing libraries were prepared from 100-250 pg DNA using the Nextera XT DNA Library Preparation kit (Illumina) according to the manufacturer's recommended protocol, with reaction volumes scaled accordingly. Batches of 24, 48, or 96 libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler. Insert sizes and concentrations for each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies).
Submitted by: Harvard T.H. Chan School of Public Health
Study: Longitudinal Multi'omics of the Human Microbiome in Inflammatory Bowel Disease
show Abstracthide Abstract
The main Inflammatory Bowel Disease (IBD) Multi'omics Database (IBDMDB) study includes multi'omics measurements from over 100 subjects, sampled biweekly over up to a year in both adult and pediatric patients with IBD (Crohn's disease and ulcerative colitis), along with non-IBD controls. Data types include fecal metagenomes, metatranscriptomes, metabolomes, and proteomes, as well as host genetics, intestinal biopsy transcriptomes, epigenetics, and 16S amplicon profiles. Subjects' medical histories and demographics are collected at baseline and medication, diet, and disease activity profiled longitudinally.
Sample: Stool sample H4042C2 WGS
SAMN07532775 • SRS2444213 • All experiments • All runs
Library:
Name: HSMA33MX_MGX
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 213,433 spots, 39.2M bases, 14.5Mb
Run# of Spots# of BasesSizePublished
SRR5950647213,43339.2M14.5Mb2017-08-22

ID:
4403021

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