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SRX3056732: GSM2730174: int-a.1 (GSHO 2055) 1327AD; Hordeum vulgare; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 19.9M spots, 2G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: RNA sequencing of Hordeum vulgare near-isogenic lines
show Abstracthide Abstract
RNA sequencing of ILs of vrs1, vrs3, vrs4 and int-c.5 in cv. Bowman Purpose: Expression analysis and variant calling Overall design: Enriched apical meristem tissue was isolated at Waddington stage (W) 3.5 and W5.0. The samples were harvested at the middle of the day, 6 to 8 h before the end of the light period.
Sample: int-a.1 (GSHO 2055) 1327AD
SAMN07444193 • SRS2402722 • All experiments • All runs
Organism: Hordeum vulgare
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Main shoot apex tissue was isolated at Waddington stage W3.5 and 5.5 and frozen in liquid nitrogen. Tissue was ground, dissolved in 500 mL TRIzol reagent (Invitrogen), incubated for 5 min at room temperature after which, 100 mL of chloroform was added. The sample was homogenized, incubated for 2 min, and centrifuged for 15 min at 4°C followed by phase separation (keeping the aqueous phase). Next, isopropanol was added and incubated for 10 min at room temperature and further purified using an RNA easy Micro Kit (Qiagen). Residual DNA was removed using a DNA-free kit (Ambion). The quality of the RNA was tested using a bio analyzer (Agilent). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM2730174
Runs: 2 runs, 19.9M spots, 2G bases, 1.3Gb
Run# of Spots# of BasesSizePublished


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