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SRX2641677: RNAseq of leaf tissue from Hordeum vulgare accession Fong Tien
1 ILLUMINA (Illumina HiSeq 2500) run: 17.9M spots, 3.6G bases, 2.5Gb downloads

Submitted by: The Sainsbury Laboratory
Study: Leaf transcriptome sequencing and assembly of several barley accessions
show Abstracthide Abstract
RNAseq was performed on several barley accessions to understand their diversity, with an emphasis on the class of plant immune receptors that contain nucleotide-binding, leucine-rich repeats (NB-LRR). The accessions were sequenced at either 100 bp or 150 bp paired end, except CI 16147 which had 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.32) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters.
SAMN06560351 • SRS2049133 • All experiments • All runs
Name: HV_06400_L1
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 17.9M spots, 3.6G bases, 2.5Gb
Run# of Spots# of BasesSizePublished


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