Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA isolation was carried out using a mixture containing a 38% v/v phenol solution saturated with 0.1 M sodium acetate , supplemented with 0.8 M guanidine thiocyanate, 0.4 M ammonium thiocyanate, 0.1 M sodium acetate, 5% v/v glycerol, 0.5% sodium lauroylsarcosine, and 5 mM EDTA. To remove polysaccharides, the Ambion Plant RNA Isolation Aid (Thermo Fisher Scientific) was added, supernatant containing RNA was purified using a Direct-zol RNA MiniPrep kit according to the manufacturer’s protocol (ZYMO RESEARCH, The Epigenetics Company, USA). RNA was Dnase treated then phenol/chloroform purified. Oligo (dT) were used to isolate mRNA. Strand-specific RNA libraries were performed at BGI TECH SOLUTIONS (HONGKONG) CO., LIMITED