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SRX1815085: GSM2184147: root, replicate B, control; Hordeum vulgare; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.7M spots, 4.7G bases, 3.3Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq (strand-specific library) of barley shoots and roots from control and heat conditions.
show Abstracthide Abstract
Samples were collected after 1 h of the heat treatment. Samples were collected for control and heat stressed plants. Overall design: RNA-seq was performed for shoot (in triplicate for each condition) and root (in duplicate for each condition)
Sample: root, replicate B, control
SAMN05195268 • SRS1477994 • All experiments • All runs
Organism: Hordeum vulgare
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA isolation was carried out using a mixture containing a 38% v/v phenol solution saturated with 0.1 M sodium acetate , supplemented with 0.8 M guanidine thiocyanate, 0.4 M ammonium thiocyanate, 0.1 M sodium acetate, 5% v/v glycerol, 0.5% sodium lauroylsarcosine, and 5 mM EDTA. To remove polysaccharides, the Ambion Plant RNA Isolation Aid (Thermo Fisher Scientific) was added, supernatant containing RNA was purified using a Direct-zol RNA MiniPrep kit according to the manufacturer’s protocol (ZYMO RESEARCH, The Epigenetics Company, USA). RNA was Dnase treated then phenol/chloroform purified. Oligo (dT) were used to isolate mRNA. Strand-specific RNA libraries were performed at BGI TECH SOLUTIONS (HONGKONG) CO., LIMITED
Experiment attributes:
GEO Accession: GSM2184147
Links:
Runs: 1 run, 23.7M spots, 4.7G bases, 3.3Gb
Run# of Spots# of BasesSizePublished
SRR361887223,724,5144.7G3.3Gb2017-02-09

ID:
2593721

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