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SRX1204335: DNase I SIM sequence data from Arabidopsis thaliana seedlings
1 ILLUMINA (Illumina HiSeq 2000) run: 156.6M spots, 3.2G bases, 2.3Gb downloads

Design: Arabidopsis seeds were surface sterilized in 12 % (w/v) bleach/0.1 % Tween 20 solution and washed extensively with sterile distilled water. Seeds were vernalized for 3 days at 4 °C in water, sown in two parallel rows on MS/agar plates (30 mM sucrose, 4.2 g Murashige and Skoog medium, and 0.8 % Phytagar, pH 5.8) covered with a 100 micron nylon membrane (Genesee Scientific). Seedlings were grown on vertical plates in the Conviron PGR15 growth chamber (12:12 h. light:dark, 21 °C, 50 % humidity, and 250 μmol/m 2 /s light intensity). Roots and leaves of 1 week old seedlings were dissected using surgical blade, flash-frozen in liquid nitrogen, and stored at −80 °C.
Submitted by: Oregon State University
Study: Arabidopsis thaliana cultivar:Col-0 Epigenomics
show Abstracthide Abstract
DNase I digested genomic DNA was isolated from leaf and root tissue using a simplified version of the DNase-Seq protocol. This data was used to map DNase hypersensitve sites (DHS) on a genome-wide scale to interrogate regions of ''open chromatin'' for its capacity to regulate gene expression.
Sample: DNase-seq library from root sample from Arabidopsis thaliana
SAMN03761933 • SRS1059404 • All experiments • All runs
Name: arab_leaf_seedling_12h
Instrument: Illumina HiSeq 2000
Strategy: DNase-Hypersensitivity
Selection: size fractionation
Layout: SINGLE
Spot descriptor:

Runs: 1 run, 156.6M spots, 3.2G bases, 2.3Gb
Run# of Spots# of BasesSizePublished


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