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SRX882899: GSM1613098: Stirling 24 h; Hordeum vulgare subsp. vulgare; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 21.3M spots, 3.8G bases, 2.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Next generation sequencing reveals gene transcription activities and alternative splicing events in germinating barley embryos
show Abstracthide Abstract
Seed germination triggers a transition of growth and metabolic activities from quiescent to active statuses. Germinating seeds is a good system to study many biological and biochemical processes including hormone metabolic activities and cell wall biosynthesis. Next generation sequence technology is used to study these processes. We have examined gene transcription activities and alternative splicing events in germinating embryos Overall design: We dissected barley embryos from four barley varieties at 2 time points 24 h and 48 h
Sample: Stirling 24 h
SAMN03352295 • SRS850269 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted and mRNA was isolated by oligodT column as described by manufactory RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1613098
External link:
Runs: 1 run, 21.3M spots, 3.8G bases, 2.5Gb
Run# of Spots# of BasesSizePublished


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