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SRX844600: GSM1585887: WT_mock_rep1; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 7.4M spots, 1.5G bases, 899.4Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: mRNA-seq of ros1-3 and ros1-4 with ABA treatment
show Abstracthide Abstract
To survey transcriptome changes by the mutations of a DNA demethylase ROS1 responding to a phytohormone abscisic acid, we performed the Next-gen sequencing (NGS) associated RNA-seq analysis. Two ROS1 knockout lines (ros1-3, ros1-4; Penterman et al. 2007 [PMID: 17409185]) with the wild-type Col line (wt) were subjected. Overall design: Three samples (ros1-3, ros1-4 and wt), biological triplicates, ABA or mock treatment, using Illumina HiSeq 2500 system
Sample: WT_mock_rep1
SAMN03285048 • SRS820503 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: For each of three replicates, five plants were pooled to make a single RNA sample. The RNA extraction steps were performed with RNeasy plant RNA extraction kit (QIAGEN) as suggested protocol. RNA libraries were prepared for the sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM1585887
Links:
External link:
Runs: 1 run, 7.4M spots, 1.5G bases, 899.4Mb
Run# of Spots# of BasesSizePublished
SRR17615067,379,9451.5G899.4Mb2016-01-15

ID:
1194448

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