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SRX689084: RNA seq of tolerant Jatropha line after infection
1 ILLUMINA (Illumina HiSeq 2000) run: 37.5M spots, 7.6G bases, 4.7Gb downloads

Design: Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 4 minutes at elevated temperature (94 o C) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments.
Submitted by: Kelkar Education Trust’s Scientific Research Centr
Study: Differentially expressed resistant reaction of Jatropha lines against Colletotrichum gloeosporioides
show Abstracthide Abstract
Jatropha genotypes 9-1 showing tolerance to Colletotrichum gloeosporioides were artificially infected with pure isolate of Colletotrichum gloeosporioides. RNA of control and induced tissue of tolerant line collected after 2hr, 24hr, 96 hr and 144 hr of infection was extracted and sequenced using NGS to analyse differentially expressed region. SNP and SSR associated in differentially expressed region will be used for mapping .
Sample: Sample B : Resistant Induced
SAMN02905750 • SRS691595 • All experiments • All runs
Organism: Jatropha curcas
Name: PoolB
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: PolyA
Layout: PAIRED
Spot descriptor:
forward102  reverse

Runs: 1 run, 37.5M spots, 7.6G bases, 4.7Gb
Run# of Spots# of BasesSizePublished


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