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SRX535360: GSM1382424: WSR2; Jatropha curcas; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 7.9M spots, 950.3M bases, 731.2Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome profiling from roots of jatropha in response to waterlogging
show Abstracthide Abstract
Gene expression analysis by high-throughtput RNA-sequencing from roots of waterlogged jatropha (Jatropha curcas). Six leaf stage seedlings (~30 d.o.) were subjected to 24 h of soil waterlogging. Overall design: 4 samples, 2 conditions (24 h nonstress and 24 h waterlogging stress) and 2 independent biological replicates of 2 mRNA pools
Sample: WSR2
SAMN02767689 • SRS603974 • All experiments • All runs
Organism: Jatropha curcas
Library:
Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Trizol (Invitrogen) RNA extraction according to manufacturer's protocol. Dnase treatment was performed on column using GF-1 RNA extraction kit (Vivantis) Poly(A)+ mRNAs were isolated from total RNAs by use of Absolutely mRNA purification kit (Agilent). Two hundred ng of poly(A)+ mRNAs was used for RNA-seq library construction with Ion Total RNA seq kit (Life Technologies).
Experiment attributes:
GEO Accession: GSM1382424
Links:
External link:
Runs: 1 run, 7.9M spots, 950.3M bases, 731.2Mb
Run# of Spots# of BasesSizePublished
SRR12754057,908,492950.3M731.2Mb2014-11-05

ID:
764911

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