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SRX047233: Illumina Transcriptome Sequencing of D. melanogster chemical treatment: Paraquat Treatment 5mM Four Day Adults
1 ILLUMINA (Illumina Genome Analyzer II) run: 75.9M spots, 15.2G bases, 9.4Gb downloads

Design: Libraries were paired-end sequenced on an Illumina Genome Analyzer II
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Drosophila melanogaster strain:y; cn bw sp Transcriptome or Gene expression
show Abstracthide Abstract
RNA-seq analysis was performed on poly(A) RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A) RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76 nt) on the GAIIx and HiSeq platforms. Fastq files were generated using pipeline version 1.5. Reads were aligned to the genome and a splice-junction database using Bowtie 0.12.0-beta1 and SPA5.pl (to identify only uniquely-mapped reads).
Sample: D. melanogster chemical treatment: Paraquat Treatment 5mM Four Day Adults
SAMN00215720 • SRS172651 • All experiments • All runs
Library:
Name: D.mel. Paraquat_5mM_AdMixed_4days
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward101  reverse

Runs: 1 run, 75.9M spots, 15.2G bases, 9.4Gb
Run# of Spots# of BasesSizePublished
SRR12425975,946,02515.2G9.4Gb2011-04-02

ID:
55561

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