barley domestication - SRA

SRX405105: barley domestication
1 ILLUMINA (Illumina HiSeq 2000) run: 12.2M spots, 2.4G bases, 1.6Gb downloads

Design: The poly-A containing mRNA was purified using poly-T oligo-attached magnetic beads, and then fragmented into small pieces. The ~180 bp fragments were used as templates to synthesize first-strand cDNA with random hexamer-primers. Second strand cDNA synthesis was performed using DNA polymerase I, dNTPs, and RNase H. The products were amplified by PCR and purified after end-repairing and adaptors ligations with the Illumina TruSeq RNA Sample preparation kit (Illumina Inc. San Diego, CA, USA).

Submitted by: ZHEJIANG UNIVERSITY

Study: Hordeum vulgare Transcriptome or Gene expression

PRJNA261456 • SRP047334 • All experiments • All runs

show Abstracthide Abstract

We hypothesized that the genome segments of cultivated barley should show certain similarity with its ancestral wild barley. Instead of whole genome sequences, we employed RNA-Seq to investigated the genomic origin of modern cultivated barley using some representative wild barley genotypes from the Near East and Tibet, and representative world-wide selections of cultivated barley.

Sample: C183

SAMN02483506 • SRS524230 • All experiments • All runs

Organism: Hordeum vulgare

Library:

Name: C183

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 12.2M spots, 2.4G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR1084227	12,240,947	2.4G	1.6Gb	2014-09-19

ID:: 580533

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity