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SRX398434: GSM1296932: H3K27me3_ChIPSeq in wt_rep1; Arabidopsis thaliana; ChIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 8.2M spots, 416.4M bases, 173.6Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Genome-wide profiling of trimethylated histone H3 lysine 27 (H3K27me3) in wide-type, brm, clf, and brm clf mutant seedlings
show Abstracthide Abstract
By using ChIP-seq, we found that loss of BRM activity in developing seedlings leads to ectopic and increased H3K27me3 deposition at several hundred genes, indicating the critical role of BRM in preventing the inappropriate deposition of this histone mark. Removal of CLF in brm mutant could partcially suppress the increased H3K27me3. Overall design: Examination of H3K27me3 in 14-day-old wt, brm, clf, and brm clf seedlings. Two biological replicates for each one.
Sample: H3K27me3_ChIPSeq in wt_rep1
SAMN02485532 • SRS518896 • All experiments • All runs
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Selection: ChIP
Layout: SINGLE
Construction protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27me3 antibody. Ten ng of ChIP DNA immuoprecipitated by anti-H3K27me3 antibody was used for ChIP-seq library construction. Blunting end, adapter ligation and amplification were carried out using Illumina Genomic DNA Sample Prep Kits according to the manufacturer's protocol. Illumina Genome Analyzer IIx was used for high-throughput sequencing of the ChIP-seq library.
Experiment attributes:
GEO Accession: GSM1296932
External link:
Runs: 1 run, 8.2M spots, 416.4M bases, 173.6Mb
Run# of Spots# of BasesSizePublished


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