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SRX392052: GSM1289430: Control biological replicate 1; Danio rerio; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 54.9M spots, 11G bases, 6.7Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Silencing of odorant receptor gene expression by G protein ß? signaling ensures the expression of one odorant receptor per olfactory sensory neuron
show Abstracthide Abstract
Olfactory sensory neurons express just one out of a possible ~1000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein ß? subunits is both necessary and sufficient to suppress the expression of odorant receptor genes, and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results provide new insights linking receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron. Overall design: Total 6 samples were analyzed-3 controls & 3 samples
Sample: Control biological replicate 1
SAMN02444384 • SRS515263 • All experiments • All runs
Organism: Danio rerio
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was harvested using Trizol reagent & DNase treated. Poly(A)+ RNA was enriched from total RNA using oligo(dT)25 magnetic beads (Life Technologies, cat#61006). The libraries were prepared using PrepX SPIA RNA-Seq (cat#400030) and ILM DNA Library kits (cat#400044) according to the manufacturer’s (IntegenX, Inc.) protocols.
Experiment attributes:
GEO Accession: GSM1289430
Links:
External link:
Runs: 2 runs, 54.9M spots, 11G bases, 6.7Gb
Run# of Spots# of BasesSizePublished
SRR104994628,888,0765.8G3.4Gb2013-12-17
SRR104994725,962,9105.2G3.3Gb2013-12-17

ID:
562899

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