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SRX391989: GSM1289373: DS22968 DNaseI-seq Tsu-1, 7 day old seedling; Arabidopsis thaliana; DNase-Hypersensitivity
2 ILLUMINA (Illumina HiSeq 2000) runs: 36.4M spots, 1.3G bases, 827.9Mb downloads

Submitted by: NCBI (GEO)
Study: Mapping and dynamics of regulatory DNA in A. thaliana accessions
show Abstracthide Abstract
We performed DNase-seq on 7-day-old seedlings from four A. thaliana accessions: Bur-0, Tsu-0, Bay-0, Est-1 using INTACT constructs (Deal and Henikoff, Developmental Cell, 2010) driven by the UBQ10 promoter. Overall design: Chromatin accessibility profiling (Dnase I-seq) of 7-day-old seedlings of A. thaliana accessions: Bur-0, Tsu-0, Bay-0 & Est-1 grown in LD conditions (16hr light 22°C, 8hr dark 20°C).
Sample: DS22968 DNaseI-seq Tsu-1, 7 day old seedling
SAMN02444258 • SRS515198 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: DNase-Hypersensitivity
Selection: DNase
Layout: SINGLE
Construction protocol: DNaseI: Nuclei were extracted and isolated with biotin-capture methods. Nuclei were then treated with 45u of DNase I for 3 minutes at 25°C. Resulting genomic DNA was size fractionated and small (double cut) fragments were sequenced using Illumina technology. Illumina TruSeq v2
Experiment attributes:
GEO Accession: GSM1289373
Runs: 2 runs, 36.4M spots, 1.3G bases, 827.9Mb
Run# of Spots# of BasesSizePublished


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