GSM1289371: DS22973 DNaseI-seq Bay-0, 7 day old seedling; Arabidopsis... - SRA

SRX391987: GSM1289371: DS22973 DNaseI-seq Bay-0, 7 day old seedling; Arabidopsis thaliana; DNase-Hypersensitivity
2 ILLUMINA (Illumina HiSeq 2000) runs: 55.9M spots, 2G bases, 1.2Gb downloads

Submitted by: NCBI (GEO)

Study: Mapping and dynamics of regulatory DNA in A. thaliana accessions

PRJNA231718 • SRP034155 • All experiments • All runs

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We performed DNase-seq on 7-day-old seedlings from four A. thaliana accessions: Bur-0, Tsu-0, Bay-0, Est-1 using INTACT constructs (Deal and Henikoff, Developmental Cell, 2010) driven by the UBQ10 promoter. Overall design: Chromatin accessibility profiling (Dnase I-seq) of 7-day-old seedlings of A. thaliana accessions: Bur-0, Tsu-0, Bay-0 & Est-1 grown in LD conditions (16hr light 22°C, 8hr dark 20°C).

Sample: DS22973 DNaseI-seq Bay-0, 7 day old seedling

SAMN02444257 • SRS515197 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Instrument: Illumina HiSeq 2000

Strategy: DNase-Hypersensitivity

Source: GENOMIC

Selection: DNase

Layout: SINGLE

Construction protocol: DNaseI: Nuclei were extracted and isolated with biotin-capture methods. Nuclei were then treated with 45u of DNase I for 3 minutes at 25°C. Resulting genomic DNA was size fractionated and small (double cut) fragments were sequenced using Illumina technology. Illumina TruSeq v2

Experiment attributes:

GEO Accession: GSM1289371

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 55.9M spots, 2G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR1049798	50,449,954	1.8G	1.1Gb	2013-12-16
SRR1049799	5,438,322	195.8M	138Mb	2013-12-16

ID:: 562830

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