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SRX043512: Illumina Transcriptome Sequencing of D. melanogster chemical treatment: Rotenone Treatment 8ug/ml Third Instar Larvae
2 ILLUMINA (Illumina Genome Analyzer II) runs: 85.5M spots, 13G bases, 5.3Gb downloads

Design: Libraries were paired-end sequenced on an Illumina Genome Analyzer II
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Drosophila melanogaster strain:y; cn bw sp Transcriptome or Gene expression
show Abstracthide Abstract
RNA-seq analysis was performed on poly(A) RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A) RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76 nt) on the GAIIx and HiSeq platforms. Fastq files were generated using pipeline version 1.5. Reads were aligned to the genome and a splice-junction database using Bowtie 0.12.0-beta1 and SPA5.pl (to identify only uniquely-mapped reads).
Sample: D. melanogster chemical treatment: Rotenone Treatment 8ug/ml Third Instar Larvae
SAMN00215718 • SRS172649 • All experiments • All runs
Library:
Name: D.mel. Rotenone_8ugperml_L3
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 2 runs, 85.5M spots, 13G bases, 5.3Gb
Run# of Spots# of BasesSizePublished
SRR10373435,873,3135.5G2.2Gb2011-02-24
SRR10373549,618,8957.5G3.2Gb2011-02-24

ID:
51784

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