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SRX381136: GSM1272460: mCherry_cntr1; Danio rerio; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 21M spots, 2.1G bases, 1.2Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Overexpression of UHRF1 drives DNA hypomethylation and hepatocellular carcinoma
show Abstracthide Abstract
UHRF1 is an essential regulator of DNA methylation that is highly expressed in many cancers. Using transgenic zebrafish, cultured cells and human tumors, we demonstrate that UHRF1 is an oncogene. RNAseq was used to assess the variation in gene expression between control and experimental samples. Overall design: Total small RNA from 2 batches of Tg(fabp10:has.UHRF1-GFP)High and age matched Tg(fabp10:nls-mCherry) control 5 dpf zebrafish livers was purified for preparation of high-throughput sequencing libraries.
Sample: mCherry_cntr1
SAMN02418768 • SRS505528 • All experiments • All runs
Organism: Danio rerio
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using Trizol and mRNA was purified using poly-A beads The mRNA was chemically fragmented,and Hi-seq compatible, bar-coded adapters were ligated to both ends.
Experiment attributes:
GEO Accession: GSM1272460
External link:
Runs: 1 run, 21M spots, 2.1G bases, 1.2Gb
Run# of Spots# of BasesSizePublished


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