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SRX361625: GSM1241788: MO_impdh1a; Danio rerio; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 35.6M spots, 1.8G bases, 1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: De Novo Purine Synthesis Mediates Circadian Control of Cell Cycle in Zebrafish (HTS)
show Abstracthide Abstract
Inosine 5''-phosphate dehydrogenase (impdh) has been well known as a key enzyme in GTP biosynthesis pathway. We found that three isoforms of impdh in zebrafish, namely impdh1a, impdh1b and impdh2, all show robust circadian expression.To examine the molecular functions of three impdh isoforms in zebrafish on the genome scale, we measured the global expression changes of impdh1a, impdh1b and impdh2 morpholino injected larvae (morphants) respectively using RNA-seq. Wild type (WT), control and three impdh morphants were collected at 32 hpf. In our RNA-seq result, we identified 468, 331 and 1166 significant genes affected by impdh1a, impdh1b and impdh2 morpholino (MO) knock-down respectively. Among them, there are limited overlaps between genes affected by different MOs and only 36 genes in common among all three MOs. This indicates that the three impdh isoforms have distinct molecular functions. Overall design: To knock down the target genes, three impdh MOs and control MO were pressure-injected into 1- to 2-cell stage embryos. WT, control and three impdh morphants were raised at 28°C under 14h: 10h light/dark cycle from birth and sampled simultaneously at 32 hpf. Each group has at least 40 embryos.
Sample: MO_impdh1a
SAMN02369772 • SRS488570 • All experiments • All runs
Organism: Danio rerio
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA of each sample was extracted using Trizol (Invitrogen).The quantity and quality of the RNA samples were assessed with a Qbit and an Agilent 2100 bioanalyzer. mRNA-seq libraries were constructed using the TruSeq RNA Sample Preparation v2 Kit following the manufacturer's protocol (Illumina).
Experiment attributes:
GEO Accession: GSM1241788
Links:
External link:
Runs: 1 run, 35.6M spots, 1.8G bases, 1Gb
Run# of Spots# of BasesSizePublished
SRR100478735,621,3051.8G1Gb2013-10-17

ID:
513293

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