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SRX008027: Illumina Transcriptome Sequencing of D. melanogaster embyros, 4-6 hour after egg laying
4 ILLUMINA (Illumina Genome Analyzer) runs: 81.4M spots, 12.4G bases, 7.1Gb downloads

Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003025 • SRS004670 • All experiments • All runs
Library:
Name: D.mel. embryos, 4-6 hr
Instrument: Illumina Genome Analyzer
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 4 runs, 81.4M spots, 12.4G bases, 7.1Gb
Run# of Spots# of BasesSizePublished
SRR02353916,796,3162.6G1.6Gb2009-09-15
SRR02401412,523,5011.9G1.1Gb2009-09-16
SRR03540526,134,1074G2.1Gb2010-02-10
SRR03540625,953,4423.9G2.3Gb2010-02-11

ID:
8306

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