U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX008018: Illumina Transcriptome Sequencing of D. melanogaster embryos, 12-14 hours after egg laying
4 ILLUMINA (Illumina Genome Analyzer) runs: 90.7M spots, 13.8G bases, 7.9Gb downloads

Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003029 • SRS004674 • All experiments • All runs
Library:
Name: D.mel. embryos, 12-14 hr
Instrument: Illumina Genome Analyzer
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 4 runs, 90.7M spots, 13.8G bases, 7.9Gb
Run# of Spots# of BasesSizePublished
SRR02360220,571,0173.1G2Gb2009-09-15
SRR02401218,924,1922.9G1.6Gb2009-09-16
SRR03539822,298,5523.4G1.8Gb2010-02-10
SRR03539928,918,0444.4G2.5Gb2010-02-11

ID:
8297

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...