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SRX008014: Illumina Transcriptome Sequencing of L3 stage larvae, light blue gut PS(3-6)
2 ILLUMINA (Illumina Genome Analyzer) runs: 15.5M spots, 2.4G bases, 1.5Gb downloads

Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003039 • SRS004687 • All experiments • All runs
Library:
Name: D.mel. L3 stage larvae, light blue gut PS(3-6)
Instrument: Illumina Genome Analyzer
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 2 runs, 15.5M spots, 2.4G bases, 1.5Gb
Run# of Spots# of BasesSizePublished
SRR0235058,354,5601.3G801.6Mb2009-09-15
SRR0271087,174,2221.1G723.6Mb2009-10-02

ID:
8293

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