U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX008169: Illumina Transcriptome Sequencing of D. melanogaster pupae, 3 days after white prepupae
2 ILLUMINA (Illumina Genome Analyzer II) runs: 29.8M spots, 2.2G bases, 1.6Gb downloads

Design: Libraries were single-end sequenced on an Illumina Genome Analyzer II in the laboratory of B. Graveley
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003086 • SRS004870 • All experiments • All runs
Library:
Name: D.mel. pupae, 3 days after WPP
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
forward

Runs: 2 runs, 29.8M spots, 2.2G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR02374412,671,586950.4M687Mb2009-09-15
SRR02378317,084,4671.3G909.1Mb2009-09-15

ID:
8455

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...