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SRX008274: Illumina Transcriptome Sequencing of D. melanogaster embyros, 8-10 hour after egg laying
1 ILLUMINA (Illumina Genome Analyzer II) run: 5M spots, 372.9M bases, 260.3Mb downloads

Design: Libraries were single-end sequenced on an Illumina Genome Analyzer II in the laboratory of B. Graveley
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003027 • SRS004672 • All experiments • All runs
Library:
Name: D.mel. embryos, 8-10 hr
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 5M spots, 372.9M bases, 260.3Mb
Run# of Spots# of BasesSizePublished
SRR0237544,972,564372.9M260.3Mb2009-09-15

ID:
8560

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