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SRX008260: Illumina Transcriptome Sequencing of adult male, 30 days after eclosion
2 ILLUMINA (Illumina Genome Analyzer II) runs: 24.6M spots, 1.9G bases, 1.2Gb downloads

Design: Libraries were single-end sequenced on an Illumina Genome Analyzer II in the laboratory of T. Gingeras
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003045 • SRS004697 • All experiments • All runs
Library:
Name: D.mel. adult male, 30 days after eclosion
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Runs: 2 runs, 24.6M spots, 1.9G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
SRR02371413,727,0471G688.9Mb2009-09-15
SRR02375210,858,848825.3M541.7Mb2009-09-15

ID:
8546

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