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SRX072621: VRC0x-like antibody sequencing
4 LS454 (454 GS FLX) runs: 1.5M spots, 580.1M bases, 345.5Mb downloads

Submitted by: VACCINE RESEARCH CENTER
Study: Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Crystal Structures and Deep Sequencing
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Antibody VRC01 represents a human immunoglobulin that neutralizes ~90% of diverse HIV-1 isolates. To understand how such broadly neutralizing HIV-1 antibodies develop and recognize the viral envelope, we used X-ray crystallography and 454 pyrosequencing to characterize additional antibodies from HIV-1-infected individuals. Crystal structures revealed a convergent mode of binding of different antibodies to the same CD4-binding-site epitope. Antibody recognition was achieved through the evolution of complementary contact domains that were generated in diverse ways. Phylogenetic analysis of expressed heavy and light chains determined by deep sequencing revealed a common pathway of antibody heavy chain maturation confined to IGHV1-2*02 lineage that could pair with different light chains. The maturation pathway inferred by antibodyomics reveals that diverse antibodies evolve to a highly affinity-matured state to recognize an invariant viral structure, providing insight into the development and evolution of broadly neutralizing HIV-1 immunity.
Sample: 454 sequencing of HIV antibodies
SAMN00622975 • SRS211654 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: VRC0X
Instrument: 454 GS FLX
Strategy: OTHER
Source: OTHER
Selection: PCR
Layout: SINGLE
Spot descriptor:
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Runs: 4 runs, 1.5M spots, 580.1M bases, 345.5Mb
Run# of Spots# of BasesSizePublished
SRR275668221,10462.7M40.8Mb2011-08-10
SRR277211697,079305M178.4Mb2011-08-10
SRR275711263,764102.9M61.3Mb2011-08-10
SRR275679305,475109.5M64.9Mb2011-08-10

ID:
82867

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