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SRX5379467: GSM3608106: Rag2null_E14_5_WholeThy_rep1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 38.2M spots, 2.7G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: Inferring population dynamics from single-cell RNA-sequencing time-series data
show Abstracthide Abstract
This dataset consists of single-cell RNA-seq (Drop-seq) data from thymi of day 14.5 mouse embryos. The sample includes the whole thymus, including mesenchyme, endothelium, epithelium, thymocytes, and other lymphocytes. The mouse is a Rag2-/- knockout. Overall design: 1 sample
Sample: Rag2null_E14_5_WholeThy_rep1
SAMN10939291 • SRS4368753 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Thymic lobes were mechanically isolated, dissociated in trypsin, depleted of RBC's (only some samples), washed and stained with 7AAD cell viability dye, and 7AAD negative single cells were sorted on a BD FACSARIA. Libraries were prepared according to the Drop-seq lab protocol version 3.1 (Macosko and Goldman, December 2015, McCarroll Lab). Final libraries were sequenced on an Illumina NextSeq 500 using high-output 75-cycle kits.
Experiment attributes:
GEO Accession: GSM3608106
Runs: 1 run, 38.2M spots, 2.7G bases, 1.3Gb
Run# of Spots# of BasesSizePublished


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