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SRX4960121: GSM3452957: E12_diencephalon; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 506.3M spots, 44.6G bases, 27Gb downloads

Submitted by: NCBI (GEO)
Study: Single-cell RNA sequencing of E12.5 mouse thalamus
show Abstracthide Abstract
In this study we performed single-cell sequencing of E12.5 mouse diencephalon revealing different newly generated neurons and their associated molecular features. Overall design: Design: Single-cell suspensions were prepared using Accumax digestion followed by manual trituration using a Pasteur pipette. Cells were processed with the 10X Genomics Chromium Single Cell Kit Version 1
Sample: E12_diencephalon
SAMN10352896 • SRS3999425 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Brains of Gbx2creER/+ embryos were dissected in ice-cold phosphate buffered saline. Under a fluorescent dissecting microscope, the thalamus, which was labeled by enhanced green fluorescent protein expressed from the Gbx2 locus, together with surrounding tissues, were dissected. Tissues from four animals were pooled, cut into pieces smaller than 1 mm in dimension, and transferred to ice-cold MACS Tissue Storage Solution (Miltenyi Biotec, Somerville, MA).  For cell dissociation, the storage solution was exchanged with RPMI 1640 Medium (Thermo-Fisher); tissue was pelleted and digested with 500 µl of pre-warmed Accumax (Innovative Cell Technologies) in a 1.5-ml tube at 37˚C for 5-10 min. At the end of digestion, the tissue pieces were dissociated by gentle trituration with a wide-bore pipet tip. The cell suspension was added to a 100-µm cell strainer (Corning, Corning, NY), and it was collected and transferred to 1.5 ml of ice-cold Resuspension Buffer (Lebovitz L15 medium with 2% FBS, 25 mM HEPES, 2 mM EDTA).  After single cell suspension, the 10X Genomics Chromium Single Cell Kit v1 (PN-120233) was used to create libraries.
Experiment attributes:
GEO Accession: GSM3452957
Runs: 1 run, 506.3M spots, 44.6G bases, 27Gb
Run# of Spots# of BasesSizePublished


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