GSM3399102: E13_cerebella; Mus musculus; RNA-Seq - SRA

SRX4739433: GSM3399102: E13_cerebella; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 490.5M spots, 43.2G bases, 27Gb downloads

Submitted by: NCBI (GEO)

Study: Single-cell RNA sequecing of E13.5 mouse cerebella

PRJNA492895 • SRP162584 • All experiments • All runs

show Abstracthide Abstract

In this study we performed single-cell sequencing of E13.5 mouse cerebella, revealing different newly generated neurons and their associated molecular features. Overall design: Design: Single-cell suspensions were prepared using Accumax digestion followed by manual trituration using a Pasteur pipette. Cells were processed with the 10X Genomics Chromium Single Cell Kit Version 1

Sample: E13_cerebella

SAMN10113640 • SRS3821006 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The cerebella were dissected from 7 E13.5 mouse embryos in ice-cold phosphate buffered saline. The dissected tissues were pooled and cut into pieces smaller than 1 mm in dimension and digested with Accumax in a 1.5-ml tube at 37˚C for 5-10 min. At the end of digestion, the tissue pieces were dissociated by gentle trituration with a wide-bore pipet tip. The cell suspension was filtered with a 100-µm cell strainer. After single cell suspension, the 10X Genomics Chromium Single Cell Kit v1 (PN-120233) was used to create libraries.

Experiment attributes:

GEO Accession: GSM3399102

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 490.5M spots, 43.2G bases, 27Gb

Run	# of Spots	# of Bases	Size	Published
SRR7988796	490,508,088	43.2G	27Gb	2019-02-19

ID:: 6422017

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