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SRX4643789: GSM3375767: tonsil CD14+ cells; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 199.3M spots, 19.5G bases, 9.3Gb downloads

Submitted by: NCBI (GEO)
Study: Single-cell RNA-seq analysis of human tonsil CD14+ cells
show Abstracthide Abstract
We performed single-cell RNA-seq on CD14+ cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology. Overall design: Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer's protocol.
Sample: tonsil CD14+ cells
SAMN09979284 • SRS3741870 • All experiments • All runs
Organism: Homo sapiens
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3' v2 Reagent Kit (10X Genomics) according to manufacturer's protocol Indexed sequencing libraries were constructed using the reagents from the Chromium Single Cell 3' v2 Reagent Kit, following these steps: (1) fragmentation, end repair and A-tailing; (2) size selection with SPRI select; (3) adaptor ligation; (4) post ligation cleanup with SPRI select; (5) sample index PCR and cleanup with SPRI select beads.
Experiment attributes:
GEO Accession: GSM3375767
Runs: 1 run, 199.3M spots, 19.5G bases, 9.3Gb
Run# of Spots# of BasesSizePublished


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