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SRX4475887: GSM3308819: 10X_Uninfected MEFs, biological replicate 1, technical replicate 1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 247.5M spots, 20.8G bases, 8.4Gb downloads

Submitted by: NCBI (GEO)
Study: Reprogram-Seq: A platform for single-cell combinatorial reprogramming [VIII]
show Abstracthide Abstract
Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest. Overall design: Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq. This series includes uninfected, non-transformed MEFs.
Sample: 10X_Uninfected MEFs, biological replicate 1, technical replicate 1
SAMN09727309 • SRS3600988 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Single cell suspension was washed with 1X PBS (0.04% BSA) before 10x Genomics procedure. The concentration of single cell suspension was adjusted to about 500 to 1000 cells/µL and was loaded on the 10x Genomics' Chromium™ system (10x Genomics, Pleasanton, CA) with the aim of 6000 to 10000 cells per channel (Chromium™ Single Cell 3' Library & Gel Bead Kit v2, catalog number 120237). Single cell RNA sequencing libraries were constructed following the manufacturer's instructions (Zheng et al., 2017).
Experiment attributes:
GEO Accession: GSM3308819
Runs: 1 run, 247.5M spots, 20.8G bases, 8.4Gb
Run# of Spots# of BasesSizePublished


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