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SRX4149408: GSM3169075: Healthy human PBMCs; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 243.3M spots, 24.6G bases, 11.2Gb downloads

Submitted by: NCBI (GEO)
Study: Single cell profiling of peripheral blood mononuclear cells from healthy human donor
show Abstracthide Abstract
Healthy human peripheral blood mononuclear cells (PBMCs) investigated with 10x Genomics single cell RNA-sequencing. Overall design: Peripheral blood mononuclear cells (PBMCs) from one healthy human donor were processed on the Chromium 3'' single cell platform (10x Genomics) and sequenced on an Illumina HiSeq 2500. FASTQ data were preprocessed using CellRanger.
Sample: Healthy human PBMCs
SAMN09291096 • SRS3363004 • All experiments • All runs
Organism: Homo sapiens
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: PBMCs were isolated from whole blood using density centrifugation. Cells were then processed by the 10X Genomics Chromium single cell platform using the manufacturer's 3' protocol to obtain a pooled library. The 10x Genomics Chromium machine partitions cells into Gel Bead-In-EMulsions (GEMs), where all generated cDNA share a common 10x Genomics barcode but uses a pool of about 750,000 barcodes to separately index each cell's transcriptome. The P7 and R2 primers were added during the GEM incubation and the P5, and R1 during library construction via end repair, A-tailing, adaptor ligation and PCR. The final libraries contained the P5 and P7 primers used in Illumina bridge amplification. The library was prepared for sequencing using an Illumina 150 cycle (v2 chemistry) high output kit and sequenced on an Illumina HiSeq 2500 to obtain paired-end reads.
Experiment attributes:
GEO Accession: GSM3169075
Runs: 1 run, 243.3M spots, 24.6G bases, 11.2Gb
Run# of Spots# of BasesSizePublished


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