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SRX3933830: GSM3094423: col680; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 129.8M spots, 12.7G bases, 7.1Gb downloads

Submitted by: NCBI (GEO)
Study: Gli1-expressing cells form the essential niche for colon stem cells and serve as a reserve Wnt-source in the small intestine
show Abstracthide Abstract
Purpose: to determine cellular heterogeneity of mesenchymal Gli1-positive cells from colon using single cell RNAse (10xGenomics); Summary: We determined 8 disctinct sub-populations of mesenchymal cells, remaining non-Gli1 epithelial cells formed a distinct separete cluster serving as a internal control of the single cells sequencing procedure Overall design: Gli1-tdTomato cells were sorted using FACS (tdTomato positvity), single cell RNA sequencing was perfomed (for details see Degirmenci et al, in prep)
Sample: col680
SAMN08930123 • SRS3166675 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: epithelial cells from Gli1-CreERT2, villin-CreERT2, lox-STOP-lox-tdTomato (24 hrs after tamoxifen) were removed by EDTA, remaining non-epithelial cells were digested with Collagenase/Dispase. Single cells mixture underwent FACS (sorting) for tdTomato-positive cells to get Gli1-tdTomato cells. These cells were further processed. Single cell RNA libraries were prepared according to the 10x Genomics protocol and sequenced on Illumina HiSeq 2500 v4 system
Experiment attributes:
GEO Accession: GSM3094423
Runs: 1 run, 129.8M spots, 12.7G bases, 7.1Gb
Run# of Spots# of BasesSizePublished


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