GSM3094423: col680; Mus musculus; RNA-Seq - SRA

SRX3933830: GSM3094423: col680; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 129.8M spots, 12.7G bases, 7.1Gb downloads

Submitted by: NCBI (GEO)

Study: Gli1-expressing cells form the essential niche for colon stem cells and serve as a reserve Wnt-source in the small intestine

PRJNA450092 • SRP139952 • All experiments • All runs

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Purpose: to determine cellular heterogeneity of mesenchymal Gli1-positive cells from colon using single cell RNAse (10xGenomics); Summary: We determined 8 disctinct sub-populations of mesenchymal cells, remaining non-Gli1 epithelial cells formed a distinct separete cluster serving as a internal control of the single cells sequencing procedure Overall design: Gli1-tdTomato cells were sorted using FACS (tdTomato positvity), single cell RNA sequencing was perfomed (for details see Degirmenci et al, in prep)

Sample: col680

SAMN08930123 • SRS3166675 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: epithelial cells from Gli1-CreERT2, villin-CreERT2, lox-STOP-lox-tdTomato (24 hrs after tamoxifen) were removed by EDTA, remaining non-epithelial cells were digested with Collagenase/Dispase. Single cells mixture underwent FACS (sorting) for tdTomato-positive cells to get Gli1-tdTomato cells. These cells were further processed. Single cell RNA libraries were prepared according to the 10x Genomics protocol and sequenced on Illumina HiSeq 2500 v4 system

Experiment attributes:

GEO Accession: GSM3094423

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 129.8M spots, 12.7G bases, 7.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR7001027	129,816,043	12.7G	7.1Gb	2018-04-17

ID:: 5395640

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