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SRX3776103: GSM3034641: Gonadal tdTomato- GFP+ cells from gonadal WAT; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 382M spots, 25.2G bases, 11.1Gb downloads

Submitted by: NCBI (GEO)
Study: Single cell RNA-sequencing of visceral adipose tissue Pdgfrß+ cells
show Abstracthide Abstract
We previously derived a doxycycline-inducible (Tet-On) lineage-tracing model that allows for the indelible labeling of Pdgfrb-expressing perivascular cells in adipose tissue of adult mice (PdgfrbrtTA; TRE-Cre; Rosa26RmT/mG; herein, “MuralChaser mice”). Prior to exposing animals to doxycyline, all cells within the stromal-vascular fraction (SVF) of adult gonadal WAT (gWAT) express membrane tdTomato from the Rosa26 locus. Following 9 days of exposure to doxycycline-containing chow diet, Cre-mediated excision of the loxP-flanked tdTomato cassette occurs in Pdgfrb-expressing cells, and membrane-bound GFP (mGFP) expression is constitutively activated. We set out to test the hypothesis that Pdgfrb-expressing perivascular cells in gonadal visceral WAT of adult mice are heterogeneous, with subpopulations harboring functionally distinct phenotypes. To this end, we performed single cell RNA-sequencing of mGFP+ cells isolated from gWAT of lean (chow fed) 8 weeks-old male MuralChaser mice following 9 days of doxycycline exposure. Overall design: We pooled tdTomato- GFP+ cells from gonadal white adipose tissue (WAT) from 5 MuralChaser mice. Single cell library preparation was performed using the 10X Genomics Single Cell 3' v2 according to the manufacturer's instructions.
Sample: Gonadal tdTomato- GFP+ cells from gonadal WAT
SAMN08663112 • SRS3030181 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The cells were isolated from gonadal WAT as previously described (Hepler et al. Elife 2017). The library was generated according to manufacturer's instructions (10X Genomics).
Experiment attributes:
GEO Accession: GSM3034641
Links:
Runs: 1 run, 382M spots, 25.2G bases, 11.1Gb
Run# of Spots# of BasesSizePublished
SRR6819250381,998,34125.2G11.1Gb2018-09-04

ID:
5206489

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