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SRX3739643: GSM3021577: Spleen CD4+ T cells_single_cells_10X; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 75.7M spots, 4.5G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)
Study: Single cell transcriptomic analysis (10x Genomics) of mouse splenic CD4+ T cells
show Abstracthide Abstract
Single cell transcriptomic analysis (10x Genomics) of mouse splenic CD4+ T cells Overall design: Single cells were sorted by flow cytometry for single cell RNAseq library preparation (10x Genomics)
Sample: Spleen CD4+ T cells_single_cells_10X
SAMN08596244 • SRS2996367 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Spleen were mechanically dissociated to single cell suspensions. CD4+ T cells were sorted by flow cytometry as DAPI- CD45+ TCRb+ CD4+ cells. Single cells were then encapsulated in nanodroplets according to the 10x Genomics protocol. RNA libraries were prepared for sequencing using standard 10X Genomics Chromium prep
Experiment attributes:
GEO Accession: GSM3021577
Links:
Runs: 1 run, 75.7M spots, 4.5G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR676853675,660,9574.5G2.3Gb2018-02-26

ID:
5156265

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