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SRX3734987: GSM3020587: IL-10-producing CD4 T cells; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 28.3M spots, 2.8G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: Single cell RNA-seq of IL-10-producing CD4 T cells during chronic LCMV infection
show Abstracthide Abstract
During chronic viral infection, the inflammatory function of CD4 T cells becomes gradually attenuated. Concurrently, Th1 cells progressively acquire the capacity to secrete the cytokine IL-10, a potent suppressor of antiviral T cell responses. To determine the transcriptional changes that underlie this T cell adaption process, we applied a single-cell RNA-sequencing approach and assessed the heterogeneity of IL-10-expressing CD4 T cells during chronic infection. Unexpectedly, our analyses revealed an IL-10-producing population with a robust Tfh-signature. Using IL-10 and IL-21 double-reporter mice, we further demonstrate that IL-10+IL-21+co-producing Tfh cells arise predominantly during chronic but not acute LCMV infection. Importantly, depletion of IL-10+IL-21+co-producing CD4 T cells or deletion of Il10 specifically in Tfh cells resulted in impaired humoral immunity and viral control. Mechanistically, B cell-intrinsic IL-10 signaling was required for sustaining germinal center reactions. Lastly, we demonstrate that IL-27 and type I IFNs differentially regulate the formation of this protective IL-10-producing Tfh subset. Thus, our findings elucidate a critical role for Tfh-derived IL-10 in promoting humoral immunity during persistent viral infection. Overall design: One sample prepared using 10x Genomics Chromium platform
Sample: IL-10-producing CD4 T cells
SAMN08581630 • SRS2991907 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: IL-10-producing (Thy1.1+) CD4 T cells were sorted by flow cytometry, then single cell libraries were prepared according the the 10x Genomics Chromium Single Cell 3' Reagent Kit protocol. IL-10-producing (Thy1.1+) CD4 T cells were sorted by flow cytometry, then single cell libraries were prepared according the the 10x Genomics Chromium Single Cell 3' Reagent Kit protocol.
Experiment attributes:
GEO Accession: GSM3020587
Links:
Runs: 1 run, 28.3M spots, 2.8G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR676378228,283,0612.8G1.3Gb2018-10-29

ID:
5151609

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