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SRX3533280: GSM2913270: V2a interneuron; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 405.1M spots, 39.7G bases, 18Gb downloads

Submitted by: NCBI (GEO)
Study: Graded arrays of spinal and supraspinal V2a interneuron subtypes underlie forelimb and hindlimb motor control [scRNA-seq]
show Abstracthide Abstract
The spinal cord contains neural networks that enable regionally-distinct motor outputs along the body axis. Nevertheless, it remains unclear how segment-specific motor computations are processed because the cardinal interneuron classes that control motor neurons appear uniform at each level of the spinal cord. V2a interneurons are essential to both fore and hindlimb movements and here we identified two major types that emerge during development: Type-I neurons marked by high Chx10 form recurrent networks with neighboring spinal neurons, and Type-II that downregulate Chx10 and project to supraspinal structures. Type-I and -II V2a interneurons are arrayed in counter-gradients and this network activates different patterns of motor output at cervical and lumbar levels. Single cell RNA-seq revealed Type-I and -II V2a neurons are each comprised of multiple subtypes. Our findings uncover a molecular and anatomical organization of V2a interneurons reminiscent of the orderly way motor neurons are divided into columns and pools. Overall design: Single-cell RNA-Seq (scRNA-Seq) conducted on cervical and lumbar spinal V2a interneurons from 2 P0 neonates. Cells were sorted via FACS and pooled as a single sample on the 10x Chromium Controller.
Sample: V2a interneuron
SAMN08294759 • SRS2808714 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Cervical and lumbar segments were dissected from 2 neonates at P0 in aCSF, and cell dissociation was conducted using Papain following the manufacturer's instruction (Worthington Biochemical) with 5% Trehalose, 50uM APV, and 800uM KA added during the dissociation process (Saxena et al., 2012). Via FACS (FACSDIVA, BD), 2800 cervical cells and 2800 lumbar cells were collected into 500ul of FACS buffer (Sternfeld et al., 201). Cell suspension was centrifuged and resuspended in 60ul of FACS buffer, 33.4ul of which was loaded onto the 10X Chromium Controller using Chromium Single Cell 3' v2 reagents. Sequencing library was prepared following the manufacturer's instructions (10X Genomics), with 12 cycles used for cDNA amplification and 11 cycles for library amplification.
Experiment attributes:
GEO Accession: GSM2913270
Runs: 1 run, 405.1M spots, 39.7G bases, 18Gb
Run# of Spots# of BasesSizePublished


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