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SRX3161834: GSM2772992: D267ALI14; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 206.4M spots, 11.8G bases, 6.7Gb downloads

Submitted by: NCBI (GEO)
Study: Single-cell RNA-seq of human airway epithelial cells
show Abstracthide Abstract
Single-cell RNA-seq of human airway epithelial cells Overall design: We have made scRNA-Seq from human airway epithelial cells after 14 days of Air-Liquid-Interface (ALI) differentiation and sequenced each cell to ~120,000 reads/cell.
Sample: D267ALI14
SAMN07606142 • SRS2492922 • All experiments • All runs
Organism: Homo sapiens
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were incubated with 0.10% protease type XIV from Streptomyces griseus in HBSS for 4 hours at 4°C degrees. Cells were detached from the Transwells by pipetting and then transferred to a microtube. 50 units of DNase I per 250µl, were directly added and cells were further incubated at room temperature for 10 min. Cells were washed in 500 µL HBSS 10% Fetal Bovine Serum then in 500 µL HBSS before being mechanically dissociated through a 26G syringe (4 times). Finally, cell suspensions were filtered through a Scienceware® Flowmi™ Cell Strainer (40µm porosity), then centrifuged (150g for 5 min) and resuspended in 500 µL of cold HBSS. Cell concentration measurements were performed and 1500 cells were used for single-cell sequencing. Single cell RNA-Seq libraries were made following the User Guide for v2 Chemistry (https://support.10xgenomics.com/single-cell/sample-prep/doc/user-guide-chromium-single-cell-3-reagent-kit-v2-chemistry). Library was sequenced as follows: 26bp Read 1 (16bp Chromium barcode and 10bp UMI), 57bp Read 2 (transcript), and 8bp i7 sample barcode for each scRNA-Seq library.
Experiment attributes:
GEO Accession: GSM2772992
Runs: 1 run, 206.4M spots, 11.8G bases, 6.7Gb
Run# of Spots# of BasesSizePublished


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