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SRX1758452: GSE81313: Homo sapiens; OTHER
31 ILLUMINA (Illumina MiSeq) runs: 62.4M spots, 29.7G bases, 15.2Gb downloads

Submitted by: NCBI (GEO)
Study: Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR
show Abstracthide Abstract
We report the discovery of T cell receptors that are present and shared in multiple HLA-matched breast cancer patient tumors using a single cell emulsion based RT-PCR technique that pairs the alpha and beta TCR chains for high throughput analysis Overall design: Analysis of TCR sequences from 20 breast cancer patient tumors and blood, as well as 6 control HLA matched individuals
Sample: p1 tumor
SAMN04978743 • SRS1434669 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina MiSeq
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: No extraction of RNA was performed, rather single cells were lysed within an emulsion droplet and RT-PCR was performed using TCR specific primers PCR amplicons were generated using primers containing the Read1, Read2, and Bridge priming sites as well as Illumina indicies (Read 2 side) and custom indicies on the Read 1 side amplicon sequencing following RT-PCR of a specific gene(s)
Experiment attributes:
GEO Accession: GSE81313
Links:
Runs: 31 runs, 62.4M spots, 29.7G bases, 15.2Gb
Run# of Spots# of BasesSizePublished
SRR35004312,430,1961.2G598Mb2016-05-15
SRR35004301,173,926588.1M277.8Mb2016-05-15
SRR3500429570,226285.9M144.2Mb2016-05-15
SRR35004287,785,5953.9G2Gb2016-05-15
SRR3500427387,070193.6M133.2Mb2016-05-15
SRR35004262,435,8181.2G603.1Mb2016-05-15
SRR35004251,598,380725.5M358.7Mb2016-05-15
SRR35004243,487,5671.6G917.2Mb2016-05-15
SRR35004232,133,0901.1G588.6Mb2016-05-15
SRR35004221,725,957864.4M434.8Mb2016-05-15
SRR35004216,682,8942.9G1.7Gb2016-05-15
SRR35004201,275,068638.6M435.5Mb2016-05-15
SRR35004192,120,6311.1G485.5Mb2016-05-15
SRR3500418922,887462.8M230.3Mb2016-05-15
SRR35004171,435,338718.6M491.5Mb2016-05-15
SRR3500416698,542349.7M165.3Mb2016-05-15
SRR3500440897,088225.1M107.6Mb2016-05-15
SRR35004392,294,1861.2G564.1Mb2016-05-15
SRR35004381,197,945600.9M281.9Mb2016-05-15
SRR35004373,372,0031.3G597.8Mb2016-05-15
SRR35004362,398,6151.2G523.2Mb2016-05-15
SRR35004352,642,0681.3G657.2Mb2016-05-15
SRR35004342,453,8821.1G550Mb2016-05-15
SRR35004332,561,1631.2G539.1Mb2016-05-15
SRR3500432924,533463.4M233.4Mb2016-05-15
SRR3500441849,739424.6M293.6Mb2016-05-15
SRR35004421,402,174703M330.7Mb2016-05-15
SRR3500443483,267242.2M165.8Mb2016-05-15
SRR35004441,422,809713.1M329.4Mb2016-05-15
SRR3500445843,551422.6M288.3Mb2016-05-15
SRR35004461,762,948883.8M403.9Mb2016-05-15

ID:
2520854

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