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SRX8472139: Hyb-Seq of Jurinea kyzylkyrensis: dried leaf
1 ILLUMINA (Illumina HiSeq 3000) run: 5.6M spots, 1.1G bases, 411.7Mb downloads

Design: We used the NEB Ultra II kit (#E7645L) with the 96 NEBNext Multiplex Oligos for Illumina primers (#E6609L). We made libraries and then pooled up to 4 libraries each for a targeted sequence capture using MycroArray baits MyBaits COS Compositae/Asteraceae 1kv1 (#305296), with a later amplification with KAPA Ready Mix and primers P5 and P7 (the reamp primers described in Meyer & Kircher 2010 (doi:10.1101:pdb.prot5448) for Illumina libraries).
Submitted by: Botanic Institute of Barcelona
Study: Generic boundaries in subtribe Saussureinae
show Abstracthide Abstract
We used a Hyb-Seq method based on a probe set of 1061 nuclear conserved orthology loci with the followingaims: (1) to obtain a well resolved phylogeny of subtribe Saussureinae with a complete genus sampling; and (2) to test the validity of the genera described or accepted in recent taxonomic treatments based on phylogenetic evidence.
Sample: Jurinea_kyzylkyrensis
SAMN15064178 • SRS6772753 • All experiments • All runs
Library:
Name: Jurinea kyzylkyrensis
Instrument: Illumina HiSeq 3000
Strategy: Targeted-Capture
Source: GENOMIC
Selection: unspecified
Layout: PAIRED
Runs: 1 run, 5.6M spots, 1.1G bases, 411.7Mb
Run# of Spots# of BasesSizePublished
SRR119263865,561,0061.1G411.7Mb2021-01-01

ID:
11012904

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