Name: Sample 3_p
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: Barley (Hordeum Vulgare cv. Barke) seeds were provided by a commercial supplier (Saatzucht J. Breun GmbH & Co. KG) and cultivated at 22/16 °C and 50 ± 5% RH at a 12/12h day/night cycle and a photon flux density of 400 μmol m-2 s-1 (µE) white light (Philips Master T Green Powers, 400 W). The preparation of epidermal peels was performed using a method developed previously (Shen et al., 2015). Epidermal Peels were collected from the abaxial side of 8 to 10 day old leaves. RNA was extracted from a total of 20 epidermal peels per sample using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). RNA isolation from whole leaves was performed equally. The extracted RNA was treated with RNase-free DNase (New England Biolabs, Ipswich, MA, USA). Quality control measurements were performed on a 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and the concentration was determined using a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). Libraries were prepared with the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) using 1 µg of RNA