U.S. flag

An official website of the United States government


Send to:

Choose Destination

ERX2085585: Illumina HiSeq 2500 paired end sequencing
1 ILLUMINA (Illumina HiSeq 2500) run: 25M spots, 7.6G bases, 3.8Gb downloads

Design: Stranded paired-end 2x150 bp
Submitted by: The James Hutton Institute, Scotland, United Kingdom (The James Hutton Institute, Scotland, United Kingd)
Study: Barley vrs3 gene - RNA-Seq
show Abstracthide Abstract
The barley inflorescence (spike) comprises a multi-noded central stalk (rachis) with tri-partite clusters of uni-floretted spikelets attached alternately along its length. Relative fertility of lateral spikelets within each cluster leads to spikes with two or six rows of grain, or an intermediate morphology. Understanding the mechanisms controlling this key developmental step could provide novel solutions to enhanced grain yield. Classical genetic studies identified five major SIX-ROWED SPIKE (VRS) genes, with four now known to encode transcription factors. Here we identify and characterise the remaining major VRS gene, VRS3, as encoding a putative Jumonji C-type H3K9me2/me3 demethylase, a regulator of chromatin state. Exploring the expression network modulated by VRS3 reveals specific interactions, both with other VRS genes and genes involved in stress, hormone and sugar metabolism. We show that combining a vrs3 mutant allele with natural six-rowed alleles of VRS1 and VRS5 leads to increased lateral grain size and greater grain uniformity.
Sample: Bw902_10_WA
SAMEA104152097 • ERS1811115 • All experiments • All runs
Organism: Hordeum vulgare
Name: unspecified
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: unspecified
Layout: PAIRED
Runs: 1 run, 25M spots, 7.6G bases, 3.8Gb
Run# of Spots# of BasesSizePublished


Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...