show Abstracthide AbstractPlants from barley cultivar Scarlett and barley Spanish landrace SBCC073 were grown until maturity in both greenhouse and growth chamber. Then, plants were irrigated every two days to maintain 70 and 20% RWC in the growth chamber and 50% RWC in the greenhouse. Samples from leaves and developing flowers were obtained after one month of treatment. RNA was obtained from 2 or 3 biological replicates of both genotypes, tissues and treatments. RNA from a total 28 samples was sequenced in a single Illumina Hiseq 2000 flowcell.